高一外研英语周报31期2022答案

25.【答案】(1)裸露的、结构简单、能够自我复制、双链环状等(答出两点即可,2分)B和C(2分)(2)HndⅢ和PI或 EcoR I和PstI(2分)驱动基因转录出mRNA(1分)(3)能吸收周围环境中DNA分子的(1分)绿(l分)(4)细菌和人体共用一套遗传密码(2分)人干扰素抗体(1分)【关键能力】本题考查考生对基因工程的理解能力和分析能力。【解题思路】(1)质粒是一种裸露的、结构简单、独立于细菌拟核DNA之外,并具有自我复制能力的很小的双链环状DNA分子。DNA复制只能从5'端到3'端,因此利用PCR技术扩增人干扰素基因时,可以从图2中A、B、C、D四种单链DNA片段中选取B和C作为引物。(2)由图可知,SmaI酶的识别序列和切割位点位于目的基因上,用该酶切割会破坏目的基因,因此对人干扰素基因片段和质粒进行酶切时,可选用限制酶HindⅢ和PatI或EoRI和PstI。基因表达载体中启动子是RNA聚合酶识别和结合的部位,能驱动基因转录出mRNA(3)用Ca处理大肠杆菌,可以使其处于能吸收周围环境中DNA的生理状态。用限制酶HndⅢ和PI或ECRI和PstI切割时会破坏红色荧光蛋白基因,但不会破坏绿色荧光蛋白基因,因此筛选含有基因表达载体的大肠杆菌时,在荧光显微镜下观察,应选择发绿色荧光的细胞。(4)因为细菌和人体共用一套遗传密码,所以人体中的基因也能在大肠杆菌中表达。要检测目的基因是否表达,常采用抗原一抗体杂交技术,要检测人干扰素基因是否表达出人干扰素,可用来检测的物质是人干扰素抗体。

书面表达One possible version:NOTICEA sports meeting will be held in the playground of our school from next Thursday to FridayAs you know, the pressure of study is very heavy now, especially for those senior 3. So the purpose of the sports meeting is to letevery student get relaxed, as a result of which we students can live happily and heal thilyEveryone is welcome to take part in it. Those who perform excellently at the sports meeting will get prizes. But don' t take theresults so serously because taking part is more important than the result. Good luck to everyone!

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